RapamycinFungusV1, Main, Exploration, bibRecord, 000797

MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells.

Identifieur interne : 000797 ( Main/Exploration ); précédent : 000796; suivant : 000798

MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells.

Auteurs : Le Guo [République populaire de Chine] ; Linlin Zhou [République populaire de Chine] ; Qian Gao [République populaire de Chine] ; Aijun Zhang [République populaire de Chine] ; Jun Wei [République populaire de Chine] ; Dantong Hong [République populaire de Chine] ; Yuankui Chu [République populaire de Chine] ; Xiangguo Duan [République populaire de Chine] ; Ying Zhang [États-Unis] ; Guangxian Xu [République populaire de Chine]

Source :

PloS one [ 1932-6203 ] ; 2017.

RBID : pubmed:28636635

Descripteurs français

KwdFr :
- Alignement de séquences (MeSH), Animaux (MeSH), Antagomirs (métabolisme), Antibactériens (pharmacologie), Autophagie (MeSH), Autophagosomes (métabolisme), Cellules RAW 264.7 (MeSH), Cysteine endopeptidases (composition chimique), Cysteine endopeptidases (génétique), Cysteine endopeptidases (métabolisme), Macrophages (cytologie), Macrophages (microbiologie), Macrophages (métabolisme), Microscopie de fluorescence (MeSH), Microscopie électronique à transmission (MeSH), Mycobacterium bovis (physiologie), Régions 3' non traduites (MeSH), Sirolimus (pharmacologie), Souris (MeSH), Séquence nucléotidique (MeSH), microARN (antagonistes et inhibiteurs), microARN (génétique), microARN (métabolisme).
MESH :
- antagonistes et inhibiteurs : microARN.
- composition chimique : Cysteine endopeptidases.
- cytologie : Macrophages.
- génétique : Cysteine endopeptidases, microARN.
- microbiologie : Macrophages.
- métabolisme : Antagomirs, Autophagosomes, Cysteine endopeptidases, Macrophages, microARN.
- pharmacologie : Antibactériens, Sirolimus.
- physiologie : Mycobacterium bovis.
- Alignement de séquences, Animaux, Autophagie, Cellules RAW 264.7, Microscopie de fluorescence, Microscopie électronique à transmission, Régions 3' non traduites, Souris, Séquence nucléotidique.

English descriptors

KwdEn :
- 3' Untranslated Regions (MeSH), Animals (MeSH), Antagomirs (metabolism), Anti-Bacterial Agents (pharmacology), Autophagosomes (metabolism), Autophagy (MeSH), Base Sequence (MeSH), Cysteine Endopeptidases (chemistry), Cysteine Endopeptidases (genetics), Cysteine Endopeptidases (metabolism), Macrophages (cytology), Macrophages (metabolism), Macrophages (microbiology), Mice (MeSH), MicroRNAs (antagonists & inhibitors), MicroRNAs (genetics), MicroRNAs (metabolism), Microscopy, Electron, Transmission (MeSH), Microscopy, Fluorescence (MeSH), Mycobacterium bovis (physiology), RAW 264.7 Cells (MeSH), Sequence Alignment (MeSH), Sirolimus (pharmacology).
MESH :
- chemical , antagonists & inhibitors : MicroRNAs.
- chemical , chemistry : Cysteine Endopeptidases.
- chemical , genetics : Cysteine Endopeptidases, MicroRNAs.
- chemical , metabolism : Antagomirs, Cysteine Endopeptidases, MicroRNAs.
- chemical , pharmacology : Anti-Bacterial Agents, Sirolimus.
- chemical : 3' Untranslated Regions.
- cytology : Macrophages.
- metabolism : Autophagosomes, Macrophages.
- microbiology : Macrophages.
- physiology : Mycobacterium bovis.
- Animals, Autophagy, Base Sequence, Mice, Microscopy, Electron, Transmission, Microscopy, Fluorescence, RAW 264.7 Cells, Sequence Alignment.

Abstract

MicroRNAs (miRNAs) are small noncoding nucleotides that play major roles in the response of host immune cells. Autophagy plays a key role in activating the antimicrobial host defense against Mycobacterium tuberculosis (M. tuberculosis). Whether miRNAs specifically influence the activation of macrophage autophagy during M. tuberculosis infection is largely unknown. In the present study, we demonstrate that Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection of macrophages leads to increased expression of miR-144-3p, which targets autophagy-related gene 4a (ATG4a), to inhibit autophagy activation and antimicrobial responses to BCG. Overexpression of miR-144-3p significantly decreased both mRNA and protein levels of ATG4a, inhibited the formation of autophagosomes in RAW264.7 cells and increased intracellular survival of BCG. However, transfection with miR-144-3p inhibitor led to an increase in ATG4a levels, accelerated the autophagic response in macrophages, and decreased BCG survival in macrophages. The experimental results of this study reveal a novel role of miR-144-3p in inhibiting autophagy activation by targeting ATG4a and enhancing BCG infection, and provide potential targets for developing improved treatment.

DOI: 10.1371/journal.pone.0179772
PubMed: 28636635
PubMed Central: PMC5479589

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000792
to stream Main, to step Curation: 000792

Le document en format XML

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<author><name sortKey="Xu, Guangxian" sort="Xu, Guangxian" uniqKey="Xu G" first="Guangxian" last="Xu">Guangxian Xu</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells.</title>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan</wicri:regionArea>
<wicri:noRegion>Yinchuan</wicri:noRegion>
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<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</nlm:affiliation>
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<author><name sortKey="Zhou, Linlin" sort="Zhou, Linlin" uniqKey="Zhou L" first="Linlin" last="Zhou">Linlin Zhou</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<wicri:noRegion>Yinchuan</wicri:noRegion>
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<affiliation wicri:level="1"><nlm:affiliation>Clinical laboratory, Affiliated Hospital of north china university of science and technology, Tangshan, China.</nlm:affiliation>
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<author><name sortKey="Gao, Qian" sort="Gao, Qian" uniqKey="Gao Q" first="Qian" last="Gao">Qian Gao</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<author><name sortKey="Zhang, Aijun" sort="Zhang, Aijun" uniqKey="Zhang A" first="Aijun" last="Zhang">Aijun Zhang</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<wicri:noRegion>Yinchuan</wicri:noRegion>
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<author><name sortKey="Wei, Jun" sort="Wei, Jun" uniqKey="Wei J" first="Jun" last="Wei">Jun Wei</name>
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<country xml:lang="fr">République populaire de Chine</country>
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<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
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<wicri:noRegion>Yinchuan</wicri:noRegion>
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<author><name sortKey="Hong, Dantong" sort="Hong, Dantong" uniqKey="Hong D" first="Dantong" last="Hong">Dantong Hong</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan</wicri:regionArea>
<wicri:noRegion>Yinchuan</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chu, Yuankui" sort="Chu, Yuankui" uniqKey="Chu Y" first="Yuankui" last="Chu">Yuankui Chu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan</wicri:regionArea>
<wicri:noRegion>Yinchuan</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Duan, Xiangguo" sort="Duan, Xiangguo" uniqKey="Duan X" first="Xiangguo" last="Duan">Xiangguo Duan</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan</wicri:regionArea>
<wicri:noRegion>Yinchuan</wicri:noRegion>
</affiliation>
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<author><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Xu, Guangxian" sort="Xu, Guangxian" uniqKey="Xu G" first="Guangxian" last="Xu">Guangxian Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan</wicri:regionArea>
<wicri:noRegion>Yinchuan</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3' Untranslated Regions (MeSH)</term>
<term>Animals (MeSH)</term>
<term>Antagomirs (metabolism)</term>
<term>Anti-Bacterial Agents (pharmacology)</term>
<term>Autophagosomes (metabolism)</term>
<term>Autophagy (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Cysteine Endopeptidases (chemistry)</term>
<term>Cysteine Endopeptidases (genetics)</term>
<term>Cysteine Endopeptidases (metabolism)</term>
<term>Macrophages (cytology)</term>
<term>Macrophages (metabolism)</term>
<term>Macrophages (microbiology)</term>
<term>Mice (MeSH)</term>
<term>MicroRNAs (antagonists & inhibitors)</term>
<term>MicroRNAs (genetics)</term>
<term>MicroRNAs (metabolism)</term>
<term>Microscopy, Electron, Transmission (MeSH)</term>
<term>Microscopy, Fluorescence (MeSH)</term>
<term>Mycobacterium bovis (physiology)</term>
<term>RAW 264.7 Cells (MeSH)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sirolimus (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Antagomirs (métabolisme)</term>
<term>Antibactériens (pharmacologie)</term>
<term>Autophagie (MeSH)</term>
<term>Autophagosomes (métabolisme)</term>
<term>Cellules RAW 264.7 (MeSH)</term>
<term>Cysteine endopeptidases (composition chimique)</term>
<term>Cysteine endopeptidases (génétique)</term>
<term>Cysteine endopeptidases (métabolisme)</term>
<term>Macrophages (cytologie)</term>
<term>Macrophages (microbiologie)</term>
<term>Macrophages (métabolisme)</term>
<term>Microscopie de fluorescence (MeSH)</term>
<term>Microscopie électronique à transmission (MeSH)</term>
<term>Mycobacterium bovis (physiologie)</term>
<term>Régions 3' non traduites (MeSH)</term>
<term>Sirolimus (pharmacologie)</term>
<term>Souris (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>microARN (antagonistes et inhibiteurs)</term>
<term>microARN (génétique)</term>
<term>microARN (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>MicroRNAs</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cysteine Endopeptidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cysteine Endopeptidases</term>
<term>MicroRNAs</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antagomirs</term>
<term>Cysteine Endopeptidases</term>
<term>MicroRNAs</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term>
<term>Sirolimus</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>3' Untranslated Regions</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>microARN</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cysteine endopeptidases</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Macrophages</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Macrophages</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cysteine endopeptidases</term>
<term>microARN</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Autophagosomes</term>
<term>Macrophages</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Macrophages</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Macrophages</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antagomirs</term>
<term>Autophagosomes</term>
<term>Cysteine endopeptidases</term>
<term>Macrophages</term>
<term>microARN</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term>
<term>Sirolimus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mycobacterium bovis</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycobacterium bovis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Autophagy</term>
<term>Base Sequence</term>
<term>Mice</term>
<term>Microscopy, Electron, Transmission</term>
<term>Microscopy, Fluorescence</term>
<term>RAW 264.7 Cells</term>
<term>Sequence Alignment</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Animaux</term>
<term>Autophagie</term>
<term>Cellules RAW 264.7</term>
<term>Microscopie de fluorescence</term>
<term>Microscopie électronique à transmission</term>
<term>Régions 3' non traduites</term>
<term>Souris</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">MicroRNAs (miRNAs) are small noncoding nucleotides that play major roles in the response of host immune cells. Autophagy plays a key role in activating the antimicrobial host defense against Mycobacterium tuberculosis (M. tuberculosis). Whether miRNAs specifically influence the activation of macrophage autophagy during M. tuberculosis infection is largely unknown. In the present study, we demonstrate that Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection of macrophages leads to increased expression of miR-144-3p, which targets autophagy-related gene 4a (ATG4a), to inhibit autophagy activation and antimicrobial responses to BCG. Overexpression of miR-144-3p significantly decreased both mRNA and protein levels of ATG4a, inhibited the formation of autophagosomes in RAW264.7 cells and increased intracellular survival of BCG. However, transfection with miR-144-3p inhibitor led to an increase in ATG4a levels, accelerated the autophagic response in macrophages, and decreased BCG survival in macrophages. The experimental results of this study reveal a novel role of miR-144-3p in inhibiting autophagy activation by targeting ATG4a and enhancing BCG infection, and provide potential targets for developing improved treatment.</div>
</front>
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<DateCompleted><Year>2017</Year>
<Month>09</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>12</Volume>
<Issue>6</Issue>
<PubDate><Year>2017</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS One</ISOAbbreviation>
</Journal>
<ArticleTitle>MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells.</ArticleTitle>
<Pagination><MedlinePgn>e0179772</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0179772</ELocationID>
<Abstract><AbstractText>MicroRNAs (miRNAs) are small noncoding nucleotides that play major roles in the response of host immune cells. Autophagy plays a key role in activating the antimicrobial host defense against Mycobacterium tuberculosis (M. tuberculosis). Whether miRNAs specifically influence the activation of macrophage autophagy during M. tuberculosis infection is largely unknown. In the present study, we demonstrate that Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection of macrophages leads to increased expression of miR-144-3p, which targets autophagy-related gene 4a (ATG4a), to inhibit autophagy activation and antimicrobial responses to BCG. Overexpression of miR-144-3p significantly decreased both mRNA and protein levels of ATG4a, inhibited the formation of autophagosomes in RAW264.7 cells and increased intracellular survival of BCG. However, transfection with miR-144-3p inhibitor led to an increase in ATG4a levels, accelerated the autophagic response in macrophages, and decreased BCG survival in macrophages. The experimental results of this study reveal a novel role of miR-144-3p in inhibiting autophagy activation by targeting ATG4a and enhancing BCG infection, and provide potential targets for developing improved treatment.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Guo</LastName>
<ForeName>Le</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Linlin</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Clinical laboratory, Affiliated Hospital of north china university of science and technology, Tangshan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gao</LastName>
<ForeName>Qian</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Aijun</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wei</LastName>
<ForeName>Jun</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hong</LastName>
<ForeName>Dantong</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chu</LastName>
<ForeName>Yuankui</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Duan</LastName>
<ForeName>Xiangguo</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Ying</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Guangxian</ForeName>
<Initials>G</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0003-3327-3315</Identifier>
<AffiliationInfo><Affiliation>Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Medical Laboratory, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>06</Month>
<Day>21</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
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<NameOfSubstance UI="D020413">3' Untranslated Regions</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000070416">Antagomirs</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance>
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<Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber>
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<Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D020413" MajorTopicYN="N">3' Untranslated Regions</DescriptorName>
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<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
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<MeshHeading><DescriptorName UI="D003546" MajorTopicYN="N">Cysteine Endopeptidases</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008264" MajorTopicYN="N">Macrophages</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
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<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
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<MeshHeading><DescriptorName UI="D035683" MajorTopicYN="N">MicroRNAs</DescriptorName>
<QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName>
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<MeshHeading><DescriptorName UI="D000067996" MajorTopicYN="N">RAW 264.7 Cells</DescriptorName>
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<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
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<MeshHeading><DescriptorName UI="D020123" MajorTopicYN="N">Sirolimus</DescriptorName>
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<ArticleIdList><ArticleId IdType="pubmed">28636635</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pone.0179772</ArticleId>
<ArticleId IdType="pii">PONE-D-16-51092</ArticleId>
<ArticleId IdType="pmc">PMC5479589</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Tissue Antigens. 2009 Sep;74(3):189-204</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19563525</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Genet. 2009;43:67-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19653858</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Autophagy. 2013 Jun 1;9(6):881-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23508006</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2013;9(10 ):e1003697</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24130493</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Death Differ. 2008 Dec;15(12):1875-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18806755</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Rev. 2007 Oct;219:37-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17850480</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2015 Jun 1;194(11):5355-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25917095</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Immunol. 2007 Oct;7(10):767-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17767194</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2004 Dec 17;119(6):753-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15607973</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Immunol. 2007 Jul;27(4):347-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17364232</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Cell Biol. 2012;2012:189794</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22536246</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomed Res Int. 2013;2013:179174</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24350246</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Nov 12;279(46):47704-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15355958</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2012 Jul 10;22(13):R540-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22790007</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Mol Biol (Noisy-le-grand). 2016 Feb 29;62(2):87-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26950457</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2011 Mar 4;286(9):7327-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21177865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2016 Mar;590(6):795-807</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26918315</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Apr 14;9(4):e94997</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24733347</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Immunol. 2016 Feb 18;7:47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26925060</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Immunol Ther Exp (Warsz). 2009 Sep-Oct;57(5):355-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19707720</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2013 Feb 15;288(7):5056-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23233675</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2008 Jun 12;3(6):399-407</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18541216</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Rev. 2013 May;253(1):112-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23550642</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2000 Nov 23;408(6811):488-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11100732</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2011 Sep 13;30(22):4628-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21915098</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2003 Feb 7;278(6):3671-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12446702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Cell Infect Microbiol. 2016 Oct 18;6:134</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27803889</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cells. 2016 Jul;39(7):543-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27329039</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Apr 22;9(4):e95716</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24755562</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(10):e47449</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23077620</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Cell Dev Biol. 2004 Apr;15(2):231-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15209383</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Hematol. 1998 Jan;5(1):72-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9515206</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Physiol Biochem. 2015;35(3):997-1007</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25660220</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immune Netw. 2011 Jun;11(3):135-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21860607</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Autophagy. 2013 Mar;9(3):374-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23322078</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Oncotarget. 2016 Mar 8;7(10):11637-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26826553</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2010 Jul;20(7):748-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20548331</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2008 Feb 28;451(7182):1069-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18305538</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbes Infect. 2008 Jul;10(9):995-1004</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18762264</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Carcinogenesis. 2012 Nov;33(11):2018-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22902544</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Infect Dis. 2016 Feb;43:68-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26724775</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Intern Med. 2013;52(20):2295-303</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24126389</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2017 Apr 5;13(4):e1006305</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28380049</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 2009;452:181-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19200883</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hippokratia. 2010 Oct;14(4):236-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21311629</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Tumour Biol. 2015 Nov 13;:null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26566625</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2007 Apr 4;26(7):1749-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17347651</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Immunol. 2013 May 06;4:97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23653625</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Mol Med. 2014 Nov;18(11):2266-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25209900</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbes Infect. 2004 Aug;6(10):946-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15310472</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Immun. 2009 May;77(5):2230-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19289514</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
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<name sortKey="Chu, Yuankui" sort="Chu, Yuankui" uniqKey="Chu Y" first="Yuankui" last="Chu">Yuankui Chu</name>
<name sortKey="Duan, Xiangguo" sort="Duan, Xiangguo" uniqKey="Duan X" first="Xiangguo" last="Duan">Xiangguo Duan</name>
<name sortKey="Gao, Qian" sort="Gao, Qian" uniqKey="Gao Q" first="Qian" last="Gao">Qian Gao</name>
<name sortKey="Guo, Le" sort="Guo, Le" uniqKey="Guo L" first="Le" last="Guo">Le Guo</name>
<name sortKey="Hong, Dantong" sort="Hong, Dantong" uniqKey="Hong D" first="Dantong" last="Hong">Dantong Hong</name>
<name sortKey="Wei, Jun" sort="Wei, Jun" uniqKey="Wei J" first="Jun" last="Wei">Jun Wei</name>
<name sortKey="Wei, Jun" sort="Wei, Jun" uniqKey="Wei J" first="Jun" last="Wei">Jun Wei</name>
<name sortKey="Xu, Guangxian" sort="Xu, Guangxian" uniqKey="Xu G" first="Guangxian" last="Xu">Guangxian Xu</name>
<name sortKey="Xu, Guangxian" sort="Xu, Guangxian" uniqKey="Xu G" first="Guangxian" last="Xu">Guangxian Xu</name>
<name sortKey="Zhang, Aijun" sort="Zhang, Aijun" uniqKey="Zhang A" first="Aijun" last="Zhang">Aijun Zhang</name>
<name sortKey="Zhou, Linlin" sort="Zhou, Linlin" uniqKey="Zhou L" first="Linlin" last="Zhou">Linlin Zhou</name>
<name sortKey="Zhou, Linlin" sort="Zhou, Linlin" uniqKey="Zhou L" first="Linlin" last="Zhou">Linlin Zhou</name>
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   |wiki=    Bois
   |area=    RapamycinFungusV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:28636635
   |texte=   MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells.
}}

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	Serveur d'exploration sur la rapamycine et les champignons
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la rapamycine et les champignons

MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells.

MicroRNA-144-3p inhibits autophagy activation and enhances Bacillus Calmette-Guérin infection by targeting ATG4a in RAW264.7 macrophage cells.

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